The Sister Cities Program is administered by Sister Cities International and was initiated by President Dwight D. Eisenhower in 1956 to encourage greater friendship and understanding between United States and other nations through direct and personal contact. The City of Tampa has formalized Sister Cities agreements with the following cities:

• Agrigento, Italy
• Ashdod, Israel
• Barranqilla, Colombia
• Boca del Rio, Mexico
• Izmir, Turkey
• LeHavre, France
• Oviedo, Spain
• Vera Cruz, Mexico